Optimization of dilute acid pretreatment of Agave lechuguilla and ethanol production by co-fermentation with Escherichia coli MM160
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Optimization of dilute acid pretreatment of Agave lechuguilla and ethanol production by co-fermentation with Escherichia coli MM160
학술지
Industrial crops and products
저자명
Dí az-Blanco, Deniss I.; de La Cruz, Jesú s R.; Ló pez-Linares, Juan C.; Morales-Martí nez, Thelma K.; Ruiz, Encarnació n; Rios-Gonzá lez, Leopoldo J.; Romero, Inmaculada; Castro, Eulogio
초록
<P><B>Abstract</B></P> <P> <I>Agave lechuguilla</I> is a common plant of Northern Mexico that can be used as feedstock in the context of a biorefinery without competition for food use. In this work, the production of fermentable sugars from this biomass has been studied for the first time using dilute sulfuric acid pretreatment. An experimental design and response surface methodology were applied with temperature (160–200 °C) and acid concentration (0.5–1.5% w/v) chosen as factors. The pretreatment conditions were expressed in a combined severity factor, which ranged from −0.75 to 2.38. According to an optimization criterion that maximizes hemicellulosic sugar recovery in the prehydrolysate and glucose recovery by enzymatic hydrolysis, optimal conditions for acid pretreatment of agave were found to be 180 °C and 1.24% (w/v) H<SUB>2</SUB>SO<SUB>4</SUB> at 10% biomass loading. These optimal conditions yielded 87% hemicellulosic sugar recovery and 68 g glucose/100 g glucose in raw agave. The whole slurry resulting from acid pretreatment of agave at optimal conditions was enzymatically saccharified yielding a sugar solution that was co-fermented by the ethanologenic <I>Escherichia coli</I> MM160. This process configuration allowed the fermentation of all sugars in raw <I>A. lechuguilla</I> in a single step reaching an ethanol yield of 73.3%.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sulfuric acid pretreatment of <I>A. lechuguilla</I> was optimized. </LI> <LI> Ethanol production by xylose-fermenting microorganisms was assessed. </LI> <LI> <I>E. coli</I> co-fermented hemicellulosic and cellulosic sugars with 73% ethanol yield. </LI> </UL> </P>