초록
<P><B>Abstract</B></P> <P>The batch fermentative hydrogen production using starch was conducted to investigate the influence of temperature (25 °C-40 °C) on the co-cultures system of <I>Bacillus cereus</I> ATCC 14579 (T) [Strain A] and <I>Brevundimonas naejangsanensis</I> BIO-TAS2-2(T) [Strain B]. The results showed that the optimal temperature for hydrogen production was 35 °C in both the sole and the mixed culture fermentation. At this temperature, the co-culturing system increased the substrate consumption rate by 34 % and 70 % compared with the Strain A and Strain B fermentation, respectively. The co-culturing system enhanced the hydrogen production yield by 52 % and 62 % compared with the Strain A and Strain B fermentation, respectively. The analysis of soluble metabolites during the fermentation process indicated that the mixed acid fermentation conducted in two strains and co-culturing system was dominated by butyric acid type fermentation at the optimal temperature. The Formate pathway probably exists in Strain A, and the PFOR pathway probably exists in Strain B. Both strains A and B play a synergistic role in hydrogen production at 35 °C using starch as substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Investigation of the temperature effect on the fermentative H<SUB>2</SUB> production by mixed cultures. </LI> <LI> Co-cultures increased the H<SUB>2</SUB> yield by 52% and 62% compared with Strain A and Strain B, respectively. </LI> <LI> H<SUB>2</SUB> production was dominated by the butyrate-type fermentation at the optimal temperature. </LI> <LI> Both strains play a synergistic role in H<SUB>2</SUB> production using starch as substrate at 35 °C. </LI> </UL> </P>