초록
<P><B>Abstract</B></P> <P> <I>Vibrio natriegens</I> is a fast-growing, non-pathogenic bacterium that is being considered as the next-generation workhorse for the biotechnology industry. However, little is known about the metabolism of this organism which is limiting our ability to apply rational metabolic engineering strategies. To address this critical gap in current knowledge, here we have performed a comprehensive analysis of <I>V. natriegens</I> metabolism. We constructed a detailed model of <I>V. natriegens</I> core metabolism, measured the biomass composition, and performed high-resolution <SUP>13</SUP>C metabolic flux analysis (<SUP>13</SUP>C-MFA) to estimate intracellular fluxes using parallel labeling experiments with the optimal tracers [1,2−<SUP>13</SUP>C]glucose and [1,6−<SUP>13</SUP>C]glucose. During exponential growth in glucose minimal medium, <I>V. natriegens</I> had a growth rate of 1.70 1/h (doubling time of 24min) and a glucose uptake rate of 3.90g/g/h, which is more than two 2-fold faster than <I>E. coli</I>, although slower than the fast-growing thermophile <I>Geobacillus</I> LC300. <SUP>13</SUP>C-MFA revealed that the core metabolism of <I>V. natriegens</I> is similar to that of <I>E. coli</I>, with the main difference being a 33% lower normalized flux through the oxidative pentose phosphate pathway. Quantitative analysis of co-factor balances provided additional insights into the energy and redox metabolism of <I>V. natriegens</I>. Taken together, the results presented in this study provide valuable new information about the physiology of <I>V. natriegens</I> and establish a solid foundation for future metabolic engineering efforts with this promising microorganism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Growth physiology and metabolism of fast-growing <I>Vibrio natriegens</I> was elucidated. </LI> <LI> <I>V. natriegens</I> has a growth rate of 1.70h<SUP>-1</SUP> in glucose minimal medium at 37°C. </LI> <LI> <SUP>13</SUP>C-flux analysis revealed that core metabolism of <I>V. natriegens</I> is similar to <I>E. coli</I>. </LI> <LI> <I>V. natriegens</I> may be a superior next-generation host for biotechnological applications. </LI> </UL> </P>