BioChem - DOS

Application of immobilized upflow anaerobic sludge blanket reactor using Clostridium LS2 for enhanced biohydrogen production and treatment efficiency of palm oil mill effluent

International journal of hydrogen energy

Singh, L.; Wahid, Z.A.; Siddiqui, M.F.; Ahmad, A.; Ab. Rahim, M.H.; Sakinah, M.

Polyethylene glycol (PEG) gel was used to immobilize hydrogen producing Clostridium LS2 bacteria for hydrogen production in an upflow anaerobic sludge blanket (UASB) reactor. The UASB reactor with a PEG-immobilized cell packing ratio of 10% weight to volume ratio (w/v) was optimal for dark hydrogen production. The performance of the UASB reactor fed with palm oil mill effluent (POME) as a carbon source was examined under various hydraulic retention time (HRT) and POME concentration. The best volumetric hydrogen production rate of 365 mL H<SUB>2</SUB>/L/h (or 16.2 mmol/L/h) with a hydrogen yield of 0.38 L H<SUB>2</SUB>/g COD<SUB>added</SUB> was obtained at POME concentration of 30 g COD/L and HRT of 16 h. The average hydrogen content of biogas and COD reduction were 68% and 65%, respectively. The primary soluble metabolites were butyric acid and acetic acid with smaller quantities of other volatile fatty acid and alcohols formed during hydrogen fermentation. More importantly, the feasibility of PEG-immobilized cell UASB reactor for the enhancement of the dark-hydrogen production and treatment of wastewater is demonstrated.

2013

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

38

5

pp.2221-2229

10.1016/j.ijhydene.2012.12.004

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65418261

Hydrogen; Polyethylene glycol (PEG); Immobilization; Upflow anaerobic sludge blanket (UASB) reactor; Palm oil mill effluent (POME)