BioChem - DOS

Acetone, butanol, and ethanol production from cane molasses using Clostridium beijerinckii mutant obtained by combined low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Li, H.g.; Luo, W.; Gu, Q.y.; Wang, Q.; Hu, W.j.; Yu, X.b.

In order to obtain mutant strains showing higher solvent tolerance and butanol production than those of wild-type strains, the butanol-producing strain Clostridium beijerinckii L175 was subjected to mutagenesis using a combined method of low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction. With this effort, mutant strain MUT3 was isolated. When it was used for butanol fermentation in P2 medium, the production of butanol was 15.8+/-0.7g/L 46% higher than the wild-type strain. Furthermore, after optimization of butanol production from cane molasses with MUT3, the maximum butanol production of 14.9+/-0.5g/L were obtained in crew-capped bottles. When ABE production by MUT3 was carried out in a bioreactor, the production of butanol and total solvent were 15.1+/-0.8g/L and 22.1+/-0.9g/L, respectively. The remarkable butanol production and solvent tolerance of MUT3 make it promising for butanol production from cane molasses.

2013

Elsevier Applied Science

0960-8524

137

pp.254-260

10.1016/j.biortech.2013.03.084

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65929475

Butanol; Clostridium beijerinckii; Low-energy ion implantation; N-methyl-N-nitro-N-nitrosoguanidine mutation; Solvent tolerance