초록
<P><B>Abstract</B></P> <P>Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium <I>Enterobacter cloacae</I> GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only <I>E</I>. <I>cloacae</I> GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC<SUB>50</SUB>) of well-known industrial strains <I>Escherichia coli</I> (24.9mM furfural) and <I>Corynebacterium glutamicum</I> (10mM furfural) based on the cell density, IC<SUB>50</SUB> of <I>E</I>. <I>cloacae</I> GGT036 (47.7mM) was significantly higher after 24h, compared to <I>E. coli</I> and <I>C. glutamicum</I>. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that <I>E. cloacae</I> GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of <I>E. cloacae</I> GGT036 was announced and this could provide an insight for engineering of <I>E. cloacae</I> GGT036 itself or other industrially relevant bacteria.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We isolated a furfural-tolerant bacterium <I>Enterobacter cloacae</I> GGT036. </LI> <LI> <I>E. cloacae</I> GGT036 showed furfural-tolerant growth under aerobic culture. </LI> <LI> IC<SUB>50</SUB> of <I>E</I>. <I>cloacae</I> GGT036 based on cell density was 47.7mM after 24h. </LI> <LI> We presented the complete genome sequence of <I>E. cloacae</I> GGT036. </LI> </UL> </P>