BioChem - DOS

Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Sasaki, D.; Sasaki, K.; Tsuge, Y.; Morita, M.; Kondo, A.

Central metabolite profiles from glucose in microbial communities during methanogenic process were compared between a stable methanogenic reactor (MR) and a deteriorated reactor (DR). The concentrations of intracellular metabolites related to the Embden-Meyerhof and pentose phosphate pathways, with the exception of pyruvate, remained high in the MR, showing increased carbon flux in the glycolysis pathway during stable methanogenesis. Extracellular acetate temporarily accumulated in the MR, consistent with higher ATP level in the MR. Intracellular concentrations of the intermediates in the reductive branch of tricarboxylic acid cycle, malate, fumarate, and succinate were higher in the DR. Low NADH/NAD<SUP>+</SUP> ratio both in the MR and DR would suggest NADH consumption during acetate and lactate/succinate production in the MR and DR, respectively. Intracellular glutamate levels were higher in the MR, correlating with lower NADPH/NADP<SUP>+</SUP> ratio concentrations in the MR. These findings contribute to a better understanding of the metabolic state during stable methanogenesis.

2014

Elsevier Applied Science

0960-8524

172

pp.83-90

10.1016/j.biortech.2014.08.054

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70482150

Metabolomic analysis; Intracellular metabolites; Central metabolism; Microbial community; Methanogenic process