초록
<P><I>Klebsiella spp</I> are one of the best natural producers of 1,3-propanediol (1,3-PD). However, their usage in the biotechnological production of the diol is limited, since the species belong to the second hazard group. Nevertheless, multiple advantageous traits of <I>Klebsiella spp</I> justify the international effort devoted to develop a biotechnological process of 1,3-PD production with these microorganisms. Apart from the process engineering approach aiming at improvement of 1,3-PD production by <I>Klebsiella spp</I>, plethora of metabolic engineering approaches have been reported. Different strategies have been undertaken to genetically improve <I>Klebsiella</I> strains and provide them with the ability to synthesize 1,3-PD more efficiently. These include over-expression of both homologous and heterologous genes of the 1,3-PD synthesis pathway, protein and cofactor engineering, deletion of the genes involved in by-products formation. This review provides an overview of the initial and most recent reports on the metabolic engineering of <I>Klebsiella spp</I> with the aim of improvement of 1,3-PD biosynthesis.</P>