BioChem - DOS

The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Harrington, T.D.; Tran, V.N.; Mohamed, A.; Renslow, R.; Biria, S.; Orfe, L.; Call, D.R.; Beyenal, H.

The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD<SUP>+</SUP> by reduced neutral red did not occur as predicted. Instead, neutral red was shown to reduce the menaquinone pool in the inner bacterial membrane. The reduced menaquinone pool altered fermentative metabolite production via the arcB redox-sensing cascade in the absence of terminal electron acceptors. When the acceptors DMSO, fumarate, or nitrate were provided, as many as 19% of the electrons trapped in the reduced acceptors were derived from the electrode. These results demonstrate the mechanism of neutral red-mediated microbial electrosynthesis during fermentation as well as how neutral red enables microbial electrosynthesis of reduced terminal electron acceptors.

2015

Elsevier Applied Science

elsevier-specific: oa user license

0960-8524

192

pp.689-695

10.1016/j.biortech.2015.06.037

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72336259

http://manuscript.elsevier.com/S0960852415008275/pdf/S0960852415008275.pdf

Neutral red; Electron transfer; Bioelectrochemical system; Microbial electrosynthesis; Menaquinone