BioChem - DOS

Multienzymatic Sucrose Conversion into Fructose and Gluconic Acid through Fed-Batch and Membrane-Continuous Processes

Applied biochemistry and biotechnology

Taraboulsi Jr., Fadi Antoine; Tomotani, Ester Junko; Vitolo, Michele

<P>Multienzymatic conversion of sucrose into fructose and gluconic acid was studied through fed-batch and continuous (in a membrane reactor) processes. The law of substrate addition (sucrose or glucose) for the fed-batch process which led to a yield superior to 80% was the decreasing linear type, whose feeding rate (?; L/h) was calculated through the equation: ? = ?(o) - k.t, where ?(o) (initial feeding rate, L/h), k (linear addition constant, L/h (2)), and t (reaction time, h). In the continuous process, the yield of conversion of sucrose (Y) was superior to 70% under the following conditions: dilution rate?=?0.33 h(-1), total duration of 15 h, pH 5.0, 37 C and initial sucrose concentration of 64 g/L (Y?=?92%), 100 g/L (Y?=?83%), or 150 g/L (Y?=?76%).</P>

2011

Springer-Verlag

0273-2289

1559-0291

165

7

pp.1708-1724

10.1007/s12010-011-9389-6

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