BioChem - DOS

Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus

Journal of agricultural and food chemistry

Ko, Jin-A; Kim, Young-Min; Ryu, Young Bae; Jeong, Hyung Jae; Park, Tae-Soon; Park, Su-Jin; Wee, Young-Jung; Kim, Joong-Su; Kim, Doman; Lee, Woo Song

<P>Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific &beta;-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50&ndash;60 &deg;C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by <SUP>1</SUP>H and <SUP>13</SUP>C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other &beta;-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 &mu;L of SSGase at pH 5.1 and 63 &deg;C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2012/jafcau.2012.60.issue-24/jf300531e/production/images/medium/jf-2012-00531e_0006.gif'></P>

2012

American Chemical Society

0021-8561

1520-5118

60

24

pp.6210-6216

10.1021/jf300531e

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63440300

stevioside; rubusoside; β-glucosidase; Aspergillus aculeatus; natural solubilizer