초록
<P><B>Abstract</B></P> <P>Low yield and inhibition of hydrogenase by oxygen are the main limitations for hydrogen production by microalgae. Considering the role of electron flow in the metabolism for hydrogen production, a genome-scale metabolic model (named <I>i</I>MM627) was reconstructed for <I>Auxenochlorella protothecoides</I>. <I>i</I>MM627 was evaluated using experimental data for growth and flux distribution. Then, considering the well-known degeneracy of FBA solutions, a new method of finding effective reactions based on multiple optimal solutions was developed. At a constant growth rate, flux distributions for maximal and minimal hydrogen production under anaerobiosis and for maximal oxygen production were compared to identify target reactions for improvement of hydrogen production and for providing anaerobiosis. Existing researches on some reactions truly confirm the predicted changes by <I>i</I>MM627. The main proposed strategies for improvement of hydrogen production include changes in metabolism to provide NADH. Consumption of oxygen by mitochondrial respiration and energy dissipation was proposed to provide anaerobiosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The first genome-scale metabolic model was reconstructed for <I>A. protothecoides.</I> </LI> <LI> The model was employed as a framework to study hydrogen and oxygen production. </LI> <LI> A new method of finding effective reactions was developed by optimal solutions. </LI> <LI> The method was applied to find strategies for improvement of hydrogen production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>