BioChem - DOS

Hyper-production of butyric acid from delignified rice straw by a novel consolidated bioprocess

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Chi, Xue; Li, Jianzheng; Wang, Xin; Zhang, Yafei; Antwi, Philip

<P><B>Abstract</B></P> <P>A novel consolidated bioprocess for hyper-production of butyric acid from delignified rice straw without exogenous enzymes involved was developed by co-fermentation of <I>Clostridium thermocellum</I> ATCC 27405 and <I>C. thermobutyricum</I> ATCC 49875. Feasibility of the consolidated bioprocess was approved by batch fermentations, with the optimum pH of 6.5. Fed-batch fermentation with a constant pH of 6.5 at 55 &deg;C could enhance the butyric acid yield to a remarkable 33.9 g/L with a selectivity as high as 78%. Metabolic analysis of the co-culture indicated that sugars liberated by <I>C. thermocellum</I> ATCC 27405 were effectively converted to butyric acid by <I>C. thermobutyricum</I> ATCC 49875. Secondary metabolism of <I>C. thermobutyricum</I> ATCC 49875 also contributed to the hyper-production of butyric acid, resulting in the re-assimilation of by-products such as acetic acid and ethanol. This work provides a more effective fermentation process for butyric acid production from lignocellulosic biomass for future applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel consolidated bioprocess was constructed for hyper-butyric acid production. </LI> <LI> <I>C. thermocellum</I> ATCC 27405 and C. thermobutyricum ATCC 49875 were co-cultured. </LI> <LI> The two strains could ferment rice straw to butyric acid production effectively. </LI> <LI> A butyric acid yield as high as 33.7 g/L was achieved with a selectivity of 78%. </LI> <LI> The bioprocess was an effective approach to lignocellulosic butyric acid production. </LI> </UL> </P>

2018

Elsevier

0960-8524

254

pp.115-120

10.1016/j.biortech.2018.01.042

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART80577888

Consolidated bioprocess; Rice straw; Butyric acid; Co-culture; Clostridia