초록
<P><B>Abstract</B></P><P>A thermophilic anaerobic bacterium <I>Clostridium</I> sp. TCW1 was isolated from dairy cow dung and was used to produce hydrogen from cellulosic feedstock. Extracellular cellulolytic enzymes produced from TCW1 strain were identified as endoglucanases (45, 53 and 70kDa), exoglucanase (70kDa), xylanases (53 and 60kDa), and β-glucosidase (45kDa). The endoglucanase and xylanase were more abundant. The optimal conditions for H<SUB>2</SUB> production and enzyme production of the TCW1 strain were the same (60°C, initial pH 7, agitation rate of 200rpm). Ten cellulosic feedstock, including pure or natural cellulosic materials, were used as feedstock for hydrogen production by <I>Clostridium</I> strain TCW1 under optimal culture conditions. Using filter paper at 5.0g/L resulted in the most effective hydrogen production performance, achieving a H<SUB>2</SUB> production rate and yield of 57.7ml/h/L and 2.03mol H<SUB>2</SUB>/mol hexose, respectively. Production of cellulolytic enzyme activities was positively correlated with the efficiency of dark-H<SUB>2</SUB> fermentation.</P>