초록
The influence of surfactant addition on the hydrogen fermentative of the organic fraction of municipal solid waste was extensively investigated under thermophilic conditions (55+/-2<SUP>o</SUP>C) in batch cultures. The addition of Tween 80® (T80) and polyethylene glycol (PEG 6000®) substantially improved hydrogen yields (HYs) resulting in 109.9+/-7.1 and 113.8+/-7.7ml<SUB>H2</SUB>/gCarb.<SUB>initial</SUB> at T80 and PEG 6000® concentrations not exceeding 2.8% and 16.7g/L, respectively. A combination of 2.8% T80 with 1.7g/L PEG 6000® achieved slightly higher HYs of 116.7+/-5.2ml<SUB>H2</SUB>/gCarb.<SUB>initial</SUB>. An artificial neural network model reliably represented the relationship between the surfactant concentration and hydrogen production with a correlation coefficient (R<SUP>2</SUP>) of 0.980. Microbial community analysis of the batches supplemented with 2.8% T80 and 1.7g/L PEG 6000® showed the dominance of the hydrogen-producing bacteria Enterobacter, Escherichia, Buttiauxella, and Pantoea. The study confirms the potential of surfactant addition for H<SUB>2</SUB> production from wastes containing organics in a particulate form.