BioChem - DOS

Effect of headspace carbon dioxide sequestration on microbial biohydrogen communities

International journal of hydrogen energy

Nasr, N.; Velayutham, P.; Elbeshbishy, E.; Nakhla, G.; El Naggar, M.H.; Khafipour, E.; Derakhshani, H.; Levin, D.B.; Hafez, H.

This study investigated the impact of CO<SUB>2</SUB> removal from the headspace of a continuous flow biohydrogen production system on the H<SUB>2</SUB> yield and microbial community structure. A comparative study was conducted in the Integrated Biohydrogen Reactor Clarifier System (IBRCS) with and without potassium hydroxide in the reactor headspace to sequester CO<SUB>2</SUB> using glucose. Headspace CO<SUB>2</SUB> sequestration increased the H<SUB>2</SUB> yield by 22% to 2.96 +/- 0.14 mol/mol<SUB>hexose</SUB>. The impact of headspace CO<SUB>2</SUB> sequestration was not limited to the improvement in H<SUB>2</SUB> yield and gas quality, as it also influenced the metabolic pathway increasing acetate concentration, and decreasing butyrate and propionate concentrations. Detailed analyses of the microbial community structure in the IBRCS before and after CO<SUB>2</SUB> sequestration revealed that removal of CO<SUB>2</SUB> from the headspace of the bioreactor had a significant impact on the microbial diversity and species distribution which rationalize the observed changes in the metabolic pathways.

2015

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

40

32

pp.9966-9976

10.1016/j.ijhydene.2015.06.077

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72399889

Biohydrogen production; Dark fermentation; Integrated biohydrogen reactor clarifier system; CO2 sequestration; Microbial community structure