Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process
Joo, Jeong Chan; Oh, Young Hoon; Yu, Ju Hyun; Hyun, Sung Min; Khang, Tae Uk; Kang, Kyoung Hee; Song, Bong Keun; Park, Kyungmoon; Oh, Min-Kyu; Lee, Sang Yup; Park, Si Jae
초록
<P><B>Abstract</B></P> <P>This study examined nine expired industrial <I>Corynebacterium glutamicum</I> strains with high lysine producing capability for enhanced production of 5-AVA. <I>C. glutamicum</I> KCTC 1857 exhibiting the highest lysine production was transformed with either original <I>Pseudomonas putida davBA</I> genes, encoding the 5-AVA biosynthesis pathway, or <I>C. glutamicum</I> codon-optimized <I>davBA</I> genes. <I>C. glutamicum</I> KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, <I>Miscanthus</I> hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51g/L 5-AVA was produced from the <I>Miscanthus</I> hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Expired industrial <I>C. glutamicum</I> strains were examined for the production 5-AVA. </LI> <LI> Recombinant <I>C. glutamicum</I> efficiently produced 5-AVA from glucose. </LI> <LI> Pretreatment process of <I>Miscanthus</I> was developed. </LI> <LI> <I>Miscanthus</I> hydrolysate solution was examined for the production of 5-AVA in recombinant <I>C. glutamicum</I>. </LI> <LI> Recombinant <I>C. glutamicum</I> efficiently produced 5-AVA from <I>Miscanthus</I> hydrolysate solution. </LI> </UL> </P>