BioChem - DOS

Homofermentative production of D-lactic acid from sucrose by a metabolically engineered Escherichia coli

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Wang, Yongze; Tian; Zhao, Jinfang; Wang, Jinhua; Yan, Tao; Xu, Liyuan; Liu, Zao; Garza, Erin; Iverson, Andrew; Manow, Ryan; Finan, Chris; Zhou, Shengde

<P>Escherichia coli W, a sucrose-positive strain, was engineered for the homofermentative production of D-lactic acid through chromosomal deletion of the competing fermentative pathway genes (adhE, frdABCD, pta, pflB, aldA) and the repressor gene (cscR) of the sucrose operon, and metabolic evolution for improved anaerobic cell growth. The resulting strain, HBUT-D, efficiently fermented 100?g?sucrose?l(-1) into 85?g?D-lactic acid?l(-1) in 72-84?h in mineral salts medium with a volumetric productivity of ~1?g?l(-1)?h(-1), a product yield of 85?% and D-lactic acid optical purity of 98.3?%, and with a minor by-product of 4?g?acetate?l(-1). HBUT-D thus has great potential for production of D-lactic acid using an inexpensive substrate, such as sugar cane and/or beet molasses, which are primarily composed of sucrose.</P>

2012

Springer-Verlag

0141-5492

1573-6776

34

11

pp.2069-2075

10.1007/s10529-012-1003-7

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