BioChem - DOS

Biosynthesis of ethyl butyrate by immobilized recombinant Rhizopus oryzae lipase expressed in Pichia pastoris

Biochemical engineering journal

Guillen, M.; Benaiges, M.D.; Valero, F.

A recombinant Rhizopus oryzae lipase expressed in Pichia pastoris has been immobilized in three different kinds of supports: EP100, Eupergit<SUP>(</SUP>R)CM and Octadecyl-Sepabeads. These immobilized derivatives have been used to catalyze the synthesis of ethyl butyrate. The effect of different parameters on initial reaction rate and yield has been studied for each biocatalyst. The variables selected for the study were: temperature, water content, shaking speed, enzyme loading, type of solvent and substrates molar ratio. The results showed the EP100 derivative as the best choice in terms of initial rate and yield but, when the residual activity as well as the reutilization capacity was studied, Octadecyl-Sepabeads exhibits the highest stability, so it was finally chosen as the best biocatalyst.

2012

Elsevier

1369-703x

65

pp.1-9

10.1016/j.bej.2012.03.009

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62369960

Rhizopus oryzae; Recombinant lipase; Flavor synthesis; Pichia pastoris; Ethyl butyrate; Immobilized lipase