BioChem - DOS

Cold active holocellulase cocktail from Aspergillus niger SH3: process optimization for production and biomass hydrolysis

Journal of the Taiwan Institute of Chemical Engineers

Tiwari, R.; Nain, P.K.S.; Singh, S.; Adak, A.; Saritha, M.; Rana, S.; Sharma, A.; Nain, L.

The conversion of cellulosic biomass into monomeric sugars is the most costly and energy intensive process in bioethanol production. Cold active cellulases produced by psychrophilic microorganisms can counter this limitation by hydrolyzing the biomass at low temperature. Enrichment of soil samples collected from the Himalayan region of India, at 5 <SUP>o</SUP>C and molecular identification revealed the prominence of phylum ascomycota. The hydrolytic potential of all isolates in terms of cellulases and xylanases were tested at different temperatures and isolate A. niger SH3 which exhibited significantly higher enzyme production at low temperature. Thereafter, experimental mixture design was employed to study the influence of different carbon sources (e.g. wheat bran, wheat straw and paddy straw). A. niger SH3 gave highest titer of endoglucanase (655.94 IU/g), &beta;-glucosidase (540.57 IU/g), FPase (31.48 IU/g) and xylanase (1951.63 IU/g) under the optimized conditions after 7 days of solid state fermentation. Hydrolysis of pretreated paddy straw using the crude hydrolytic enzymes (15 FPU/gds) of A. niger SH3 at 40 <SUP>o</SUP>C resulted in maximum sugar yield (375.84 +/- 0.23 mg/gds). This study proves the potential of A. niger SH3 secretome for efficient biomass hydrolysis at low temperature and its suitability for developing an energy efficient biofuel production process.

2015

Elsevier BV

1876-1070

1876-1089

56

pp.57-66

10.1016/j.jtice.2015.04.026

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73401469

Solid state fermentation; Holocellulase; Saccharification; Experimental mixture design; Secretome