BioChem - DOS

Use of evolutionary operation (EVOP) factorial design technique to develop a bioprocess using grease waste as a substrate for lipase production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kumar, S.; Katiyar, N.; Ingle, P.; Negi, S.

The aim of the present work was to develop a bioprocess using EVOP-factorial design technique employing grease waste as a substrate for the production of lipase. A newly isolated fungal strain of Penicillium chrysogenum was explored for the fermentation process. Solid-state fermentation (SSF) was carried out using grease waste and Czapek-dox medium, supplemented with wheat bran. The yield of lipase was 38U/ml when SSF was carried out at 32<SUP>o</SUP>C for 8days and grease:wheat bran:Czapek-dox media in 1:1:2 (w/w/v). Different physicochemical parameters affecting the production of lipase were optimized through evolutionary operation (EVOP) factorial design technique and after optimization yield was enhanced up to 46U/ml at 30<SUP>o</SUP>C, pH 7.0 with 1:1:2 (w/w/v) grease waste:wheat bran:Czapek-dox media. Industrial grease waste has never been reported before for the production of industrially important lipase enzyme.

2011

Elsevier Applied Science

0960-8524

102

7

pp.4909-4912

10.1016/j.biortech.2010.12.114

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55883686

Solid-state fermentation; EVOP; Lipase; Grease waste; Penicillium chrysogenum