BioChem - DOS

Compact multi-enzyme pathways in P. pastoris

Chemical communications : Chem comm

Geier, Martina; Fauland, Pia; Vogl, Thomas; Glieder, Anton

<P>We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.</P><BR><BR><P>Graphic Abstract</P><P>2A peptides enabling the coordinate expression of nine genes in <I>P. pastoris</I> represent a valuable tool for pathway construction and engineering.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc08502g'><BR></P>

2015

The Royal Society of Chemistry

1359-7345

1364-548x

51

9

pp.1643-1646

10.1039/c4cc08502g

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71353980

https://pubs.rsc.org/en/content/articlepdf/2015/cc/c4cc08502g