This paper describes the development of a new bifunctional catalyst system that integrates enzyme and chemical material into a biochemical composite through a stepwise crosslinking approach. The as-prepared biochemical composite not only allows ''one-pot'' biomass conversion via sequential enzyme-catalyzed hydrolysis of biomass materials to glucose and metal-catalyzed hydrogenation of glucose to sorbitol, but also enables reusability of the catalyst. This design concept facilitates access to fuels and chemicals from the biomass-derived sorbitol and will attract more attention in the foreseeable future.