Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli

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논문

Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli

학술지

Organic process research & development

저자명

Dascier, Dimitri; Kambourakis, Spiros; Hua, Ling; Rozzell, J. David; Stewart, Jon D.

초록

This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a bis-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully.

발행연도

2014

발행기관

American Chemical Society

ISSN

1083-6160

ISSN

1520-586x

권

호

페이지

pp.793-800