BioChem - DOS

Integrating an algal β-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Chang, Jui-Jen; Thia, Caroline; Lin, Hao-Yeh; Liu, Hsien-Lin; Ho, Feng-Ju; Wu, Jiunn-Tzong; Shih, Ming-Che; Li, Wen-Hsiung; Huang, Chieh-Chen

<P><B>Abstract</B></P> <P>The algal &beta;-carotene hydroxylase gene <I>Crchyb</I> from <I>Chlamydomonas reinhardtii</I>, <I>Czchyb</I> from <I>Chlorella zofingiensis</I>, or <I>Hpchyb</I> from <I>Haematococcus pluvialis</I> and six other carotenoid-synthesis pathway genes were co-integrated into the genome of a yeast host. Each of these three algal genes showed a higher efficiency to convert &beta;-carotene to downstream carotenoids than the fungal genes from <I>Phaffia rhodozyma</I>. Furthermore, the strain with <I>Hpchyb</I> displayed a higher carotenoid productivity than the strains integrated with <I>Crchyb</I> or <I>Czchyb</I>, indicating that <I>Hpchyb</I> is more efficient than <I>Crchyb</I> and <I>Czchyb</I>. These results suggest that &beta;-carotene hydroxylase plays a crucial role in the biosynthesis of carotenoids.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Carotenoid biosynthesis genes from green algae. </LI> <LI> A designed carotenoid biosynthesis pathway in yeast. </LI> <LI> Bioengineering approaches to reducing manufacturing cost. </LI> </UL> </P>

2015

Elsevier

0960-8524

184

pp.2-8

10.1016/j.biortech.2014.11.097

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71650636

Green algae; Carotenoids; β-Carotene hydroxylase; Synthetic biology