초록
<P>Chlorogenic acid, a pharmacologically important compound, is a phenolic compound that occurs in certain commonly used medicinal herbs. We looked for the presence of this compound in the callus cultures of <I>Varthemia persica</I> DC (var. <I>persica</I>). We have evaluated the conditions for establishment of callus cultures of <I>V. persica</I> and the <I>in vitro</I> production of chlorogenic acid. Callus was initiated by culturing seedling of <I>V. persica</I> on MS basal medium supplemented with different concentrations of kinetin, naphthalene acetic acid and 2,4-diphenoxy acetic acid. Also, the influence of light, and phytohormones on the production of chlorogenic acid was examined. Kinetin stimulated the production of chlorogenic acid. Replacement of 2,4-diphenoxy acetic acid with naphthalene acetic acid did not alter the chlorogenic acid production. The ability to induce the accumulation of chlorogenic acid in the <I>V. persica</I> callus cultures offers an opportunity to produce a phenolic compound with therapeutic value.</P>