BioChem - DOS

Enzymatic polymerization of polythiophene by immobilized glucose oxidase

Polymer

Krikstolaityte, V.; Kuliesius, J.; Ramanaviciene, A.; Mikoliunaite, L.; Kausaite-Minkstimiene, A.; Oztekin, Y.; Ramanavicius, A.

In this study 'green', environmentally friendly enzymatic reaction-based synthesis of conducting polymer polythiophene (PTP) is proposed. Glucose oxidase (GO<SUB>x</SUB>) was shown as an effective catalyst, which, in the presence of glucose, produces hydrogen peroxide suitable for the oxidative polymerization of PTP under ambient conditions at neutral pH. Enzymatically induced formation of the PTP layer over GO<SUB>x</SUB>-modified graphite rod electrode (GRE) was demonstrated and evaluated amperometrically and by attenuated total reflectance - Fourier transform infrared (ATR-FTIR) spectroscopy. Surface morphology of GO<SUB>x</SUB>- and PTP-modified GR electrodes was characterized by atomic force microscopy. It was clearly shown that the apparent kinetic Michaelis constant (K<SUB>M(app.)</SUB>) of GO<SUB>x</SUB>/PTP-modified GRE increased by increasing the duration of polymerization reaction. Therefore, enzymatic polymerization could be applied in adjustment and/or tuning of K<SUB>M(app.)</SUB> and other kinetic parameters of GO<SUB>x</SUB>-based electrodes used in biosensor design.

2014

Butterworth Scientific Ltd ; Elsevier Science Ltd., [etc.]

0032-3861

1873-2291

55

7

pp.1613-1620

10.1016/j.polymer.2014.02.003

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68835590

Conducting polymers; Polythiophene; Enzymatic polymerization