초록
<P><B>Abstract</B></P><P>In an era of ever‐increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as <I>Yarrowia lipolytica</I> to convert waste materials into biofuels. Here, we constructed two <I>Y. lipolytica</I> strains that displayed both increased lipid accumulation and more efficient use of biomass‐derived sugars, including glucose, fructose, galactose and inulin. The first strain, <I>Y. lipolytica</I> YLZ150, was derived from the French wild‐type strain W29. It had inhibited triacylglycerol mobilization (<I>∆tgl4</I>) and <I>β</I>‐oxidation (<I>∆pox1–6</I>), and it overexpressed <I>GPD1</I>, <I>DGA2</I>, <I>HXK1</I>, the native Leloir pathway, <I>SUC2</I> from <I>Saccharomyces cerevisiae</I> and <I>INU1</I> from <I>Kluyveromyces marxianus</I>. The second strain, <I>Y. lipolytica</I> Y4779, was derived from the Polish A‐101 strain. It had inhibited <I>β</I>‐oxidation <I>(∆mfe2</I>) and overexpressed <I>GPD1</I>, <I>DGA1</I>, <I>HXK1</I>, <I>YHT3, SUC2</I> and <I>INU1</I>. In the first experiment, strain YLZ150 was batch‐cultured in media containing different hexoses; the highest values for lipid concentration and yield of lipids from the substrate were obtained using fructose (20.3 g dm<SUP>−3</SUP> and 0.14 g g<SUP>−1</SUP>, respectively). In the second experiment, we grew the two strains in fed‐batch cultures to examine lipid biosynthesis from inulin (a fructose polymer). For Y4779, the lipid concentration was 10.3 g dm<SUP>−3</SUP> and the yield of lipids from substrate was 0.07 g g<SUP>−1</SUP>; in contrast, for YLZ150, these values were 24 g dm<SUP>−3</SUP> and 0.16 g g<SUP>−1</SUP>, respectively. The YLZ150 strain is thus able to efficiently exploit glucose, fructose, galactose, sucrose and inulin for lipid biosynthesis. Copyright © 2017 John Wiley & Sons, Ltd.</P>