초록
<P><I>Nannochloropsis</I> is a genus of unicellular eukaryotes known primarily from the marine environment whose members are potential sources of lipids and long‐chain polyunsaturated fatty acids; for the extraction of these and other valuable cell components, cell disruption is needed. High pressure homogenization (HPH) would be particularly suitable for microalgae with a recalcitrant cell wall such as <I>Nannochloropsis</I>. HPH conditions should be determined based on both the target cell component and properties of the cell suspension that in some cases are dependent on the age of the culture. The yields of soluble protein and total sugars from <I>N. oculata</I> ranged from 22.7 to 50.4 mg/g and from 55.0 to 62.5 mg/g, respectively, depending on HPH conditions (loading pressure and number of passes). The yield of the lipids extracted with the method of Bligh and Dyer was not affected by HPH conditions whereas lipids extracted with Soxhlet method ranged between 8.2 and 16.2%. Main fatty acids in the lipids extracted with the method of Bligh and Dyer and total lipids were palmitic acid (17.2 ± 0.1–23.0 ± 0.2%), palmitoleic acid (22.9 ± 0.3–19.1 ± 0.9%), and eicosapentaenoic acid (20.6 ± 0.3–29.2 ± 0.3%). HPH of <I>N. oculata</I> cells promoted a different effect on particle size distribution (PSD) depending on the age of the culture. HPH reduced cell aggregation observed in the 10 day cell suspension, whereas it promoted aggregation of the 30 day cell suspension.</P><P><B>Practical applications</B>: The feasibility of producing a wide range of products from microalgae is determined by the culture conditions and the conditions of the stages in the downstream processing. Because main microalgae components are intracellular, a scalable cell disruption operation such as HPH is required. Cell disruption degree is determined not only by the equipment design and its operational conditions but also by the cell suspension properties. The results allowed us to conclude that a different combination loading pressure/number of passes in HPH maximizes the recovery of hydrosoluble compounds (proteins and sugars) and lipids in <I>N. oculata</I>. Besides, since the PSD of the microalgae suspension is a function of culture age, this variable could affect process productivity.</P><P>The effects of HPH conditions on the subsequent recovery of hydrosoluble intracellular components (protein and sugars) and the extraction of lipids accumulated by <I>N. oculata</I> were determined. The best combination of the operation conditions for HPH was used for the recovery of the studied components from the microalgae harvested at different periods (culture age). Particle size distribution (PSD) of the cell suspension before and after homogenization was determined to test the effect of culture age on cell disruption. Our results suggest that the conditions of HPH of <I>N. oculata</I> should be defined considering the component of interest.</P>