초록
<P><B>Abstract</B></P><P>Treatment with NaHSO<SUB>3</SUB> induces a 10-fold increase in H<SUB>2</SUB> photoproduction in the filamentous N<SUB>2</SUB>-fixing cyanobacterium <I>Anabaena</I> sp. strain PCC 7120. However, it is unclear whether this treatment also increases H<SUB>2</SUB> photoproduction in green alga. In this study, treatment with 13mM NaHSO<SUB>3</SUB> resulted in about a 200-fold increase in H<SUB>2</SUB> production in <I>Chlamydomonas reinhardtii</I>, and this increase was most probably the result of reduced O<SUB>2</SUB> content and enhanced hydrogenase activity. Compared to the conventional strategy of sulfur deprivation, NaHSO<SUB>3</SUB> treatment results in a higher maximum rate of H<SUB>2</SUB> photoproduction, greater efficiency of conversion of light energy into H<SUB>2</SUB>, shorter half-time to produce the maximum accumulated H<SUB>2</SUB> levels, and reduced costs because no centrifugation is involved. We therefore conclude that NaHSO<SUB>3</SUB> treatment is an efficient, rapid, and economic strategy for improving photobiological H<SUB>2</SUB> production in the green alga <I>C. reinhardtii</I>.</P>