초록
<P><B>Abstract</B></P> <P>In this work, new halophyte plant biomass is proposed as raw material for bioenergy production. The bioconversion process of lignocellulosic materials into ethanol needs a pretreatment before enzymatic hydrolysis of vegetal material to increase the yield of fermentable sugars. Various mechanical and chemical pre-treatment processes were investigated in an attempt to facilitate the solubilization of large fraction of hemicelluloses.</P> <P>The fungi <I>Trichoderma</I> spp., <I>Aspergillus niger</I>, <I>Penicillium italicum</I> and <I>Fusarium</I> spp. have been developed on the halophyte plant, <I>Juncus maritimes</I>, producing polysaccharides hydrolases activities, i.e. endoglucanasecatalyses cellulose hydrolysis, and beta-glucosidasecatalyses hydrolysis of terminal non-reducing residues in beta-glucosides. The saccharification was carried out with an enzymatic preparation extracted from filamentous fungus <I>Trichoderma</I> spp. Experimental Doehlert design was performed to optimize the reducing sugars concentration. High sugar yields (8.5？g/L) were obtained using a small amount of the extracted enzyme without stirring.</P> <P>The highest enzymatic activities of endoglucanase and beta-glucosidases were produced by <I>Trichoderma</I> spp. at 22.12 and 0.07？U/mg, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A combined pretreatment was performed on <I>Retama retam.</I> </LI> <LI> The enzymatic hydrolysis were performed by a preparation from <I>Trichoderma</I> spp. </LI> <LI> The hydrolysis yield is about 25.8%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>