BioChem - DOS

An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli

Process biochemistry

Tan, J.S.; Ling, T.C.; Mustafa, S.; Tam, Y.J.; Ramanan, R.N.; Ariff, A.B.

A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97g/L) and α-PrIFN-2b production (867.4μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively.

2013

Elsevier Applied Science

1359-5113

48

4

pp.551-558

10.1016/j.procbio.2013.02.024

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66030647

http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf

Bioreactors; Expanded bed adsorption; Fermentation; Acetic acid; α-Interferon-2b