BioChem - DOS

Coupled CO2 fixation from ethylene oxide off-gas with bio-based succinic acid production by engineered recombinant Escherichia coli

Biochemical engineering journal

Wu, M.; Zhang, W.; Ji, Y.; Yi, X.; Ma, J.; Wu, H.; Jiang, M.

Industrial exhaust gases contributed heavily to anthropogenic CO<SUB>2</SUB> emissions. In this study, a novel approach of capturing CO<SUB>2</SUB> from ethylene oxide off-gas and converting it into a platform C4 chemical was established by using succinate-producing E. coli. Fermentation results showed that the acetaldehyde in the off-gas was the main factor inhibiting cell metabolic capability, thus IrrE which is a global regulator from Deinococcus radiodurans was used to improve cell resistance to acetaldehyde. A final succinic acid titer of 68.12gL<SUP>-1</SUP> with a CO<SUB>2</SUB> fixation rate of 4.7mmolL<SUP>-1</SUP>h<SUP>-1</SUP> was achieved. Notably, this coupled process not only realized the CO<SUB>2</SUB> fixation from ethylene oxide off-gas but also provided a potential cost-effective gas source which holds potential application for bio-based succinate production.

2017

Elsevier

1369-703x

117

1

pp.1-6

10.1016/j.bej.2016.07.019

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76988328

Ethylene oxide exhaust; CO2 fixation; Succinic acid; IrrE; E. coli