Optimization of culture media for ethanol production from glycerol by Escherichia coli
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Optimization of culture media for ethanol production from glycerol by Escherichia coli
학술지
Biomass & bioenergy
저자명
Cofre, O.; Ramirez, M.; Gomez, J.M.; Cantero, D.
초록
The culture media for glycerol to ethanol biotransformation by Escherichia coli under anaerobic conditions was optimized. A Plackett-Burman screening design was used to determine which factors were significant with a 95% confidence interval. A full factorial 2<SUP>2</SUP> and response surface model were employed to determine the optimum conditions for the selected response variable. The response variable considered in this work was biomass productivity (Q<SUB>X</SUB>). Profiles for biomass growth, glycerol consumption and ethanol production were obtained at optimum conditions and fermentation parameters were calculated. Glycerol to ethanol yield and ethanol specific productivity were determined to be 845 g kg<SUP>-1</SUP> of glycerol and 212 g kg<SUP>-1</SUP> h<SUP>-1</SUP> of cell mass respectively. Optimized culture media is presented along with the main results and experimental profiles obtained from this work.