BioChem - DOS

In situ laccase treatment enhances the fermentability of steam-exploded wheat straw in SSCF processes at high dry matter consistencies

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Moreno, A.D.; Tomas-Pejo, E.; Ibarra, D.; Ballesteros, M.; Olsson, L.

This work evaluates the in situ detoxification of inhibitory lignocellulosic broths by laccases to facilitate their fermentation by the xylose-consuming Saccharomyces cerevisiae F12. Treatment of wheat straw slurries with laccases prior to SSCF processes decreased the total phenolic content by 50-80%, reducing the lag phase and increasing the cell viability. After laccase treatment, a negative impact on enzymatic hydrolysis was observed. This effect, together with the low enzymatic hydrolysis yields when increasing consistency, resulted in a decrease in final ethanol yields. Furthermore, when using high substrate loading (20% DM (w/v)), high concentration of inhibitors prevailed in broths and the absence of an extra nitrogen source led to a total cell growth inhibition within the first 24h in non-treated samples. This inhibition of growth at 20% DM (w/v) was overcome by laccase treatment with no addition of nitrogen, allowing S. cerevisiae F12 to produce more than 22g/L of ethanol.

2013

Elsevier Applied Science

0960-8524

143

pp.337-343

10.1016/j.biortech.2013.06.011

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66421662

Lignocellulose; Bioethanol; Simultaneous saccharification and co-fermentation; In situ laccase detoxification; S. cerevisiae F12