BioChem - DOS

Use of dry-milling derived thin stillage for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Liang, Y.; Zhao, X.; Strait, M.; Wen, Z.

This study was to explore the use of thin stillage, a major byproduct in dry milling corn-ethanol plants, for production of eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. Thin stillage contains various compounds that were ideal for fungal growth. Thin stillage concentration and temperature played important roles in fungal growth and EPA production. When 50% thin stillage was used in a stepwise temperature shift culture process, the cell density reached 23g/L at day 9 with EPA yield and productivity of 243 and 27mg/Lday, respectively. The fungal biomass contained 39% lipid, 28% protein, 30% carbohydrate, and 3% ash. The fungal culture also generated a nutrient-depleted liquid by removing organic compounds in the raw thin stillage. The results collectively showed a new use of thin stillage by feeding to the fungus P. irregulare for producing omega-3 fatty acids.

2012

Elsevier Applied Science

0960-8524

111

pp.404-409

10.1016/j.biortech.2012.02.035

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62205148

Pythium irregulare; Dry milling; Thin stillage; Fungal fermentation; Eicosapentaenoic acid