BioChem - DOS

Highly efficient methane biocatalysis revealed in a methanotrophic bacterium

Nature communications

Kalyuzhnaya, M. G.; Yang, S.; Rozova, O. N.; Smalley, N. E.; Clubb, J.; Lamb, A.; Gowda, G. A. Nagana; Raftery, D.; Fu, Y.; Bringel, F.; Vuilleumier, S.; Beck, D. A. C.; Trotsenko, Y. A.; Khmelenina, V. N.; Lidstrom, M. E.

Methane is an essential component of the global carbon cycle and one of the most powerful greenhouse gases, yet it is also a promising alternative source of carbon for the biological production of value-added chemicals. Aerobic methane-consuming bacteria (methanotrophs) represent a potential biological platform for methane-based biocatalysis. Here we use a multi-pronged systems-level approach to reassess the metabolic functions for methane utilization in a promising bacterial biocatalyst. We demonstrate that methane assimilation is coupled with a highly efficient pyrophosphate-mediated glycolytic pathway, which under oxygen limitation participates in a novel form of fermentation-based methanotrophy. This surprising discovery suggests a novel mode of methane utilization in oxygen-limited environments, and opens new opportunities for a modular approach towards producing a variety of excreted chemical products using methane as a feedstock.

2013

Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

2041-1723

4

pp.2785

10.1038/ncomms3785

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68313643

https://www.nature.com/articles/ncomms3785.pdf