초록
<P><B>Abstract</B></P> <P>In this study, the combined ionizing radiation-acid pretreatment process was firstly applied to enhance hydrogen fermentation of grass waste. Results showed that the combined pretreatment synergistically enhanced hydrogen fermentation of grass waste. The SCOD and soluble polysaccharide contents of grass waste increased by 1.6 and 2.91 times after the combined pretreatment, respectively. SEM observation and crystallinity test showed the combined pretreatment effectively disrupted the grass structure. Owing to the more favorable substrate conditions, the hydrogen yield achieved 68 mL/g-dry grass<SUB>added</SUB> after the combined pretreatment, which was 161.5%, 112.5% and 28.3% higher than those from raw, ionizing radiation pretreated and acid pretreated grass waste, respectively. The VS removal also increased from 13.9% to 25.6% by the combined pretreatment. Microbial community analysis showed that the abundance of dominant hydrogen producing genus <I>Clostridium sensu stricto 1</I> increased from 37.9% to 69.4% after the combined pretreatment, which contributed to more efficient hydrogen fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Combined radiation-acid pretreatment was applied to enhance H<SUB>2</SUB> fermentation. </LI> <LI> Noticeable dissolution and disruption of grass waste occurred after pretreatment. </LI> <LI> H<SUB>2</SUB> yield of grass waste increased by 161.5% after the combined pretreatment. </LI> <LI> H<SUB>2</SUB> yield achieved 68 mL/g-grass<SUB>added</SUB> after the combined pretreatment. </LI> <LI> More enrichment of <I>Clostridium </I>contributed to more efficient H<SUB>2</SUB> fermentation. </LI> </UL> </P>