Biomimicry Promotes the Efficiency of a 10-Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오플라스틱
  1. 플라스틱
- 바이오정밀화학
  1. 기타
- 화장품용 기능성소재
  1. 기능성
  2. 계면활성제⁄증점제
- 의료용 화학소재
  1. 치료제
  2. 건강보조식품
  3. 식품첨가제

논문

Biomimicry Promotes the Efficiency of a 10-Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate

학술지

Angewandte Chemie

저자명

Mukai, Chinatsu; Gao, Lizeng; Nelson, Jacquelyn L.; Lata, James P.; Cohen, Roy; Wu, Lauren; Hinchman, Meleana M.; Bergkvist, Magnus; Sherwood, Robert W.; Zhang, Sheng; Travis, Alexander J.

초록

AbstractFor nanobiotechnology to achieve its potential, complex organic–inorganic systems must grow to utilize the sequential functions of multiple biological components. Critical challenges exist: immobilizing enzymes can block substrate‐binding sites or prohibit conformational changes, substrate composition can interfere with activity, and multistep reactions risk diffusion of intermediates. As a result, the most complex tethered reaction reported involves only 3 enzymes. Inspired by the oriented immobilization of glycolytic enzymes on the fibrous sheath of mammalian sperm, here we show a complex reaction of 10 enzymes tethered to nanoparticles. Although individual enzyme efficiency was higher in solution, the efficacy of the 10‐step pathway measured by conversion of glucose to lactate was significantly higher when tethered. To our knowledge, this is the most complex organic–inorganic system described, and it shows that tethered, multi‐step biological pathways can be reconstituted in hybrid systems to carry out functions such as energy production or delivery of molecular cargo.

발행연도

2017

ISSN

0044-8249

ISSN

1521-3757

권

129

호

페이지

pp.241-244

DOI