BioChem - DOS

Fermentative H2 production using a switchgrass steam exploded liquor fed to mixed anaerobic cultures: Effect of hydraulic retention time, linoleic acid and nitrogen sparging

International journal of hydrogen energy

Veeravalli, S.S.; Chaganti, S.R.; Lalman, J.A.; Heath, D.D.

Fermentative hydrogen (H<SUB>2</SUB>) production from a steam exploded switchgrass liquor using inhibited mixed anaerobic microbial communities was studied in upflow anaerobic sludge blanket reactors (UASBRs). Increasing the H<SUB>2</SUB> yield was accomplished by treating the inoculum with linoleic acid (LA), varying the hydraulic retention time (HRT) and sparging liquid phase with nitrogen (N<SUB>2</SUB>). A maximum H<SUB>2</SUB> yield of 2.56 +/- 0.10 mol mol<SUP>-1</SUP> hexose, was obtained at a 6 h HRT in LA treated cultures sparged with N<SUB>2</SUB>. Sparging or LA treatment alone was able to enhance the H<SUB>2</SUB> yield by 46 +/- 5% and 38 +/- 3%, respectively, in comparison to control cultures operating at a 6 h HRT. Of the different methods employed, N<SUB>2</SUB> sparging in combination with LA treatment proved to be more effective in enriching the H<SUB>2</SUB> producing bacteria belonging to Clostridium sp. Species belonging to Propionibacterium, Bacteroides and Eubacterium, which were associated with H<SUB>2</SUB> consumption and reduced byproducts formation, were observed in addition to Clostridium sp. in unsparged control cultures.

2014

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

39

19

pp.9994-10002

10.1016/j.ijhydene.2014.04.114

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69194752

Mixed anaerobic cultures; Hydraulic retention time; Nitrogen sparging; Switchgrass; Biohydrogen production