BioChem - DOS

Exploiting Acid Phosphatases in the Synthesis of Phosphorylated Monoalcohols and Diols

European journal of organic chemistry

Tasná di, Gá bor; Lukesch, Michael; Zechner, Michaela; Jud, Wolfgang; Hall, Mé lanie; Ditrich, Klaus; Baldenius, Kai; Hartog, Aloysius F.; Wever, Ron; Faber, Kurt

<P><B>Abstract</B></P><P>A set of phosphatases was evaluated for their potential to catalyze the regio&#x2010; and stereoselective phosphorylation of alcohols using a high&#x2010;energy inorganic phosphate donor, such as di&#x2010;, tri&#x2010; and polyphosphate. Parameters such as type and amount of phosphate donor and pH of the reaction were investigated in order to minimize the thermodynamically favored hydrolysis of the phosphate donor and the formed phosphate ester. Diols were monophosphorylated with high selectivities. This biocatalytic phosphorylation method provides selectively activated and/or protected synthetic intermediates for further chemical and/or enzymatic transformations and is applicable to a large scale (6.86 g) in a flow setup with immobilized phosphatase.</P>

2016

WILEY&#x2010;VCH Verlag

1434-193x

1099-0690

2016

1

pp.45-50

10.1002/ejoc.201501306

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74766188

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ejoc.201501306