초록
Na<SUB>3</SUB>PO<SUB>4</SUB> and Na<SUB>2</SUB>S were employed as efficient alkaline catalysts for the pretreatment of corn stover. To systematically obtain optimal conditions, the effects of critical pretreatment parameters including sodium phosphate concentration (1-4%), sulfidity (0-20%), pretreatment temperature (100-120<SUP>o</SUP>C), and reaction time (20-60min) on the reducing sugar yield of pretreated substrates were evaluated in a lab-scale using the response surface methodology. Pretreated under the sodium phosphate concentration of 4%, sulfidity of 10%, temperature of 120<SUP>o</SUP>C, and reaction time of 40min, the reducing sugar yield and glucose yield of the pretreated corn stover achieved 91.11% and 64.01%, respectively, with a moderate enzyme loading of 30FPU/g substrate. Additionally, a strong correlation (R<SUP>2</SUP>=0.971 and R<SUP>2</SUP>=0.954) between the delignification and the reducing sugar yield (or glucose yield) was observed by this pretreatment method. These results evidently support that the combined Na<SUB>3</SUB>PO<SUB>4</SUB>-Na<SUB>2</SUB>S pretreatment is an effective and feasible method for processing lignocellulosic biomass.