초록
Ethanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H<SUB>2</SUB>SO<SUB>4</SUB>, 2% NaOH and 2% NaOH in H<SUB>2</SUB>O<SUB>2</SUB> presoaking at room temperature for 24hours. Pretreated OPF by presoaking in 2% H<SUB>2</SUB>SO<SUB>4</SUB>, 2% NaOH and 2% NaOH in H<SUB>2</SUB>O<SUB>2</SUB> contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H<SUB>2</SUB>SO<SUB>4</SUB>, 2% NaOH and 2% NaOH in H<SUB>2</SUB>O<SUB>2</SUB> presoaking was 45.72, 55.73 and 56.94g/l, respectively. Ethanol concentration of 2% H<SUB>2</SUB>SO<SUB>4</SUB>, 2% NaOH and 2% NaOH in H<SUB>2</SUB>O<SUB>2</SUB> presoaking was 14.5, 15.0 and 17.2g/L, respectively. 2% NaOH in H<SUB>2</SUB>O<SUB>2</SUB> presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%.