BioChem - DOS

Biological abatement of cellulase inhibitors

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cao, Guangli; Ximenes, Eduardo; Nichols, Nancy N.; Zhang, Leyu; Ladisch, Michael

<P><B>Abstract</B></P> <P>Removal of enzyme inhibitors released during lignocellulose pretreatment is essential for economically feasible biofuel production. We tested bio-abatement to mitigate enzyme inhibitor effects observed in corn stover liquors after pretreatment with either dilute acid or liquid hot water at 10% (w/v) solids. Bio-abatement of liquors was followed by enzymatic hydrolysis of cellulose. To distinguish between inhibitor effects on enzymes and recalcitrance of the substrate, pretreated corn stover solids were removed and replaced with 1% (w/v) Solka Floc. Cellulose conversion in the presence of bio-abated liquors from dilute acid pretreatment was 8.6% (0.1x enzyme) and 16% (1x enzyme) higher than control (non-abated) samples. In the presence of bio-abated liquor from liquid hot water pretreated corn stover, 10% (0.1x enzyme) and 13% (1x enzyme) higher cellulose conversion was obtained compared to control. Bio-abatement yielded improved enzyme hydrolysis in the same range as that obtained using a chemical (overliming) method for mitigating inhibitors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bio-abatement removes enzyme inhibitors from biomass liquors. </LI> <LI> Cellulose conversion in bio-abated vs. non-abated liquors increased 1.2 to 1.5-fold. </LI> <LI> Bio-abatement gives similar improvement in cellulose conversion as overliming. </LI> <LI> Bio-abatement is a promising method to detoxify lignocellulosic slurries. </LI> </UL> </P>

2013

Elsevier

0960-8524

146

pp.604-610

10.1016/j.biortech.2013.07.112

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66953231

Bio-abatement; Inhibitors; Biomass liquor; Enzymatic hydrolysis