New contributions for industrial n-butanol fermentation: An optimized Clostridium strain and the use of xylooligosaccharides as a fermentation additive
메타 데이터
바이오화학분류
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
논문
New contributions for industrial n-butanol fermentation: An optimized Clostridium strain and the use of xylooligosaccharides as a fermentation additive
<P><B>Abstract</B></P> <P>n-Butanol is a chemical widely used in industry, with an approximate annual output of 4 million tons and a market valued at USD 8.6 billion. It was first produced by ABE fermentation (acetone - n-butanol - ethanol) at the beginning of the twentieth century using molasses as a substrate, an expensive raw material that made the process economically unfeasible. In this work, we developed a second-generation fermentation process using cellulosic sugar as the substrate. A strain of <I>Clostridium saccharoperbutylacetonicum</I> has been evolved to n-butanol resistance, and we have found that the addition of xylooligosaccharides to the culture medium leads to a significant increase in the n-butanol yield and productivity. As a result, an n-butanol titer of 12.5 g/L and a productivity of 0.43 g/L.h were obtained, a respective gain of 17% and 58% relative to the initial process. These advances indicate that second-generation technologies can make viable the production of renewable n-butanol.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>C. saccharoperbutylacetonicum</I> produces n-butanol from sugarcane straw hydrolysate. </LI> <LI> Adaptive evolution is an efficient tool to develop n-butanol tolerant strains. </LI> <LI> Cellulose pulp can be added in the fermentation to improve bacterial performance. </LI> <LI> Xylooligosaccharides can act as a prebiotic stimulating the bacterial metabolism. </LI> </UL> </P>