초록
Clostridium thermocellum ATCC 27405 was used to degrade sugarcane bagasse (SCB) directly for hydrogen production, which was significantly enhanced by supplementing medium with CaCO<SUB>3</SUB>. The effect of CaCO<SUB>3</SUB> concentration on the hydrogen production was investigated. The hydrogen production was significantly enhanced with the CaCO<SUB>3</SUB> concentration increased from 10mM to 20mM. However, with the CaCO<SUB>3</SUB> concentration further increased from 20mM to 100mM, the hydrogen production didn't increase further. Under the optimal CaCO<SUB>3</SUB> concentration of 20mM, the hydrogen production reached 97.83+/-5.19mmol/L from 2% sodium hydroxide-pretreated SCB, a 116.72% increase over the control (45.14+/-1.03mmol/L), and the yield of hydrogen production reached 4.89mmol H<SUB>2</SUB>/g SCB<SUB>added</SUB>. Additionally, CaCO<SUB>3</SUB> promoted the biodegradation of SCB and the growth of C. thermocellum. The stimulatory effects of CaCO<SUB>3</SUB> on biohydrogen production are mainly attributed to the buffering capacity of carbonate. The study provides a novel strategy to enhance biohydrogen production from lignocellulose.