초록
<P><B>Abstract</B></P> <P>Cellulose, the most abundant biopolymer on earth, is produced at different ratios by all land plants. Since the morphology and crystallinity of cellulose are key factors involved in its enzymatic hydrolysis, in the present work, we tackled the study of the effects of such variables on the nanocellulose conversion into glucose. Cellulase from <I>Trichoderma sp</I> at 37 °C was used to produce glucose, the best results were found for the cellulose nanoplatelets (S-CNP) after 60 h of hydrolysis, which afforded a conversion of 47% to glucose, in contrast to 15% for the non-purified sample (W-CP) and 22% for microcrystalline cellulose (MCC20) used as control. The X-ray diffractogram recorded on the samples showed an initial crystallinity index of 45%, 54% and 72% for W-CNP, S-CNP and MCC20, respectively. Also, we showed that after 24 h of hydrolysis, long cellulose nanofibrils (∅ ≈ 30 nm) were found as a residue.</P> <P><B>Highlights</B></P> <P> <UL> <LI> One-pot hydrolysis to obtain cellulose nanoplatelets ≈80 nm thick is described. </LI> <LI> Nanofibrils of ∅ ≈ 30 nm were used to extract cellulose fibrils ∅ ≈ 3 nm. </LI> <LI> The enzymatic hydrolysis of nanocellulose to obtain sugars is described. </LI> <LI> Cellulose nanofibrils were obtained as a residue of the saccharification. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>