초록
<P>The use of purified and overexpressed alcohol dehydrogenases to synthesize enantiopure fluorinated alcohols is shown. When the bioreductions were performed with ADH-A from <I>Rhodococcus ruber</I> overexpressed in <I>E. coli</I>, no external cofactor was necessary to obtain the enantiopure (<I>R</I>)-derivatives. Employing <I>Lactobacillus brevis</I> ADH, it was possible to achieve the synthesis of enantiopure (<I>S</I>)-fluorohydrins at a 0.5 M substrate concentration. Furthermore, due to the activated character of these substrates, a huge excess of the hydrogen donor was not necessary.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/joceah/2013/joceah.2013.78.issue-14/jo400962c/production/images/medium/jo-2013-00962c_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jo400962c'>ACS Electronic Supporting Info</A></P>