초록
<P><I>Talaromyces cellulolyticus</I> (formerly known as <I>Acremonium cellulolyticus</I>) is one of the high cellulolytic enzyme-producing fungi. <I>T. cellulolyticus</I> exhibits the potential ability for high amount production of enzyme proteins. Using the homologous expression system under the control of a glucoamylase promoter, some kinds of cellulases of <I>T. cellulolyticus</I> can be expressed by <I>T. cellulolyticus</I>. On the other hand, hyperthermophilic cellulase has been expected to be useful in the industrial applications to biomass. The hyperthermophilic archaea <I>Pyrococcus horikoshii</I> and <I>P. furiosus</I> have GH family 5 and 12 hyperthermophilic endocellulase, respectively. The two kinds of hyperthermophilic endocellulases were successfully produced by <I>T. cellulolyticus</I> using the above expression system under the control of a glucoamylase promoter of <I>T. cellulolyticus</I>. These recombinant cellulases exhibited the same characteristics as those of the recombinant cellulases prepared in <I>E. coli.</I> The productions of the recombinant enzymes were estimated to be over 100 mg/L. In this study, we first report the overexpression of the hyperthermophilic enzymes of archaea using the fungal expression system.</P>