BioChem - DOS

Liquefaction of sugarcane bagasse for enzyme production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cunha, F.M.; Kreke, T.; Badino, A.C.; Farinas, C.S.; Ximenes, E.; Ladisch, M.R.

The objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by Aspergillus niger. After initial solid state fermentation of steam pretreated bagasse solids by A. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30+/-0.07Pas at 30% (w/v) solids. Addition of A. niger for submerged fermentation of sterile liquefied bagasse at 23% w/v solids resulted in an enzyme titer of 2.5IUmL<SUP>-1</SUP> or about 15x higher productivity than solid-state fermentation of non-liquefied bagasse (final activity of 0.17IUmL<SUP>-1</SUP>). Bagasse not treated by initial solid-state fermentation but liquefied with enzyme gave 2IUmL<SUP>-1</SUP>. These results show the utility of liquefied bagasse as a culture medium for enzyme production in submerged fermentations.

2014

Elsevier Applied Science

publisher-specific-oa

0960-8524

172

pp.249-252

10.1016/j.biortech.2014.09.025

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70482245

Liquefaction; Sugarcane bagasse; Cellulase production; Fermentation; Aspergillus niger